Question

After the demonstration of totipotency, a botanist wishes to produce identical plants from an angiosperm by tissue culture. The parts of tissues, which are used in tissues culture are
A. Stem apices
B. Roots tips
C. Tissues from phloem
D. Anthers

Answer 1

Hint: Totipotency is when a plant contains all the qualities and his capacity to develop from a cell to full plant.

Complete Answer:
Steward et al took the 2mg. cuts of phloem tissue of carrot (Daucus carrots) and developed in a fluid supplement medium containing coconut water. The aspect of the plant taken for tissue culture is known as Explants (Ex. Phloem tissue of Carrot root). The fluid supplement medium containing explants was permitted a few hours for shaking.

At that point the culture cell isolates persistently to shape the mass of undifferentiated tissue known as callus. A portion of these cell groups began separating the underlying roots. At the point when it moves to a semi strong medium then it creates a shoot framework and gives rise to new plants. These plants at that point move to pot/soil where they create into a streaming plant.Plant tissue determined structure root, stem, leaf, vegetative bud, botanical bud, anther and embryo can show cell totipotency.

So, the correct answer is option C.

Note: F. C. Steward and his associates at Cornell University of USA, contrived a technique for developing carrot tissue by extracting little disc, from the sec­ondary phloem locale of carrot root and putting them in a moving fluid medium under aseptic conditions. In the presence of coconut milk in the medium, the phloem tissue started to develop effectively.