Question

A chimeric DNA is formed by
a. Deleting selectable markers
b. Joining cDNA with plasmid
c. EcoRI
d. Enzyme beta-galactosidase

Answer 1

Hint: The word chimeric derived from the Greek word chimaera, which means having different sets of DNA, which is done by joining two distinct DNA sources.

Complete answer:
Chimeric DNA is nothing but forming DNA with a different fragment from different sources. The DNA made by ligating the fragments from different sources is nothing but hybrid DNA, to say that it is a mix of DNA fragments from 2 sources

It involves several steps to form the chimeric DNA includes
- Isolation of DNA
- Digestion of DNA with restriction enzymes
- DNA fragments are joined
- Inserting DNA into host cells
- Screening and selection of transformed cells

For vectors, we mostly use plasmids which are round in shape or small circular, and it has the capability to replicate independently or self-replicating.
So it is formed by joining the plasmid DNA with a complementary DNA fragment.
From the above explanation, the correct option is joining the cDNA ( complementary DNA) with plasmid.

Additional information:
Chimeric or recombinant DNA not only occurs in eukaryotes, in prokaryotes sometimes the particular type of recombination occurs called conjugation. In both prokaryotes and eukaryotes, recombination plays a significant role in the repair of the DNA

Note: Chimeric DNA is also called recombinant DNA So it shouldn’t confuse, and they are nothing but combining different DNA fragments of different species.