Question

Write a full form of RFLP.

Answer 1

Hint: RFLP was one of the first molecular markers discovered. They were created for the purpose of genetic mapping. According to the principle of RFLP markers, any genomic DNA can be differentiated based on the presence or absence of restriction enzyme sites in DNA.

Complete answer:
In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that uses polymorphisms in homologous DNA sequences to identify individuals, populations, or species or to pinpoint the locations of genes within a sequence. The term may refer to a polymorphism detected by varying the locations of restriction enzyme sites, or it may refer to both.

Restriction fragment length polymorphisms is the full name of RFLP. The term may refer to a polymorphism in and of itself, as distinguished by the contrasting areas of limitation chemical locales, or to a related research center procedure that can delineate such contrasts. In an RFLP investigation, a DNA test is divided into sections by at least one limitation catalyst, and the subsequent parts are then isolated by gel electrophoresis as indicated by their size.

Although it is now largely obsolete due to the advancement of reasonable DNA sequencing innovations, RFLP examination was the first DNA profiling procedure that was sufficiently inexpensive to see the broad application. RFLP testing was a significant early tool in genome planning, quality restriction for hereditary issues, risk assessment for illness, and paternity testing.


Note: The fundamental method for identifying RFLPs entails dividing a sample of DNA with the use of a limitation chemical, which can specifically separate a DNA atom anywhere a short, explicit arrangement is perceived in a cycle known as a limitation digest.