Question

Which of the following phase is liquid in liquid chromatography
A. Mobile phase
B. Stationary phase
C. Both A and B
D. None of the above

Answer 1

Hint:-Liquid chromatography is the process by which a mixture of liquid Is separated into its individual components. The liquid chromatography can separate only a mixture of two immiscible liquids. Chromatography works on the principle of adsorption.

Complete answer:-The liquid chromatography Involves two phases- the stationary phase and the mobile phase. Liquid chromatography is based on the principle that the sample has to cross through a polar mobile phase and a non-polar stationary phase.
The stationary phase involves solid medium through which the measure has to pass while the mobile phase involves liquid medium. The solid mediums used are polymers and silica. These are porous and allow the liquid mixture to pass through them.
The separation is based on the affinity of the Compounds in the solvent towards the solid material in the stationary phase and the solvent. The component of the mixture which has the greatest affinity towards the solid packing is the last to come out of the column. Greater the affinity of the compound towards the packing material greater is the time it takes to move out of the column.
Examples of liquid chromatography are High-Performance Liquid Chromatography (HPLC), liquid-liquid chromatography (LLC), size exclusion chromatography, and supercritical fluid chromatography.
Thus, the right option is C.

Note:- - The gas chromatography is another type of chromatography. A mixture of nitrogen and helium gas can be separated using gas chromatography.
- Paper chromatography is used to separate mixtures like colours from dyes.
- There are four types of chromatography namely, liquid chromatography, gas chromatography paper chromatography, and thin-layer chromatography.
- Approximately, it takes 10 to 60 minutes for liquid chromatography to completely separate the components of a liquid mixture.