Answer
Verified
372.6k+ views
Hint:Recombinant DNA technology is the process of joining two DNA fragments from two species and insertion of this combined DNA into host for multiplication and production of desired products .It has several steps and the final step is down stream processing.
Complete answer:
Downstream processing refers to the process of purification and separation of products of recombinant DNA technology. Suitable preservatives are added to the product .Proper quality check is maintained. In the case of drugs and medicines proper clinical trials are conducted. Pure homogeneous products are obtained which are free of other molecules. After all this desired quality products are obtained and released into the market for public usage.
Process of downstream processing are :
1.Removal of insolubles: obtaining particulate free liquid using filtration , centrifugation, sedimentation , precipitation etc.
2.Product isolation: Removal of substances having different properties than desired product using ultrafiltration , solvent extraction etc.
3.Product purification: separation of substances having very close chemical and physical properties with desired product using chromatography and crystallization.
4.Product polishing: packaging of product.
Additional Information:
Recombinant DNA technology consists of steps such as:
1.Isolation of genetic material.
2.Cutting of DNA at specific sites with the help of restriction enzymes.
3.Extraction and amplification of genes of interest.
4.Insertion of recombinant DNA in the host cell.
5.Downstream processing.
Note:
Downstream streaming is the last part of recombinant DNA technology which includes purification and separation of the desired product.
Complete answer:
Downstream processing refers to the process of purification and separation of products of recombinant DNA technology. Suitable preservatives are added to the product .Proper quality check is maintained. In the case of drugs and medicines proper clinical trials are conducted. Pure homogeneous products are obtained which are free of other molecules. After all this desired quality products are obtained and released into the market for public usage.
Process of downstream processing are :
1.Removal of insolubles: obtaining particulate free liquid using filtration , centrifugation, sedimentation , precipitation etc.
2.Product isolation: Removal of substances having different properties than desired product using ultrafiltration , solvent extraction etc.
3.Product purification: separation of substances having very close chemical and physical properties with desired product using chromatography and crystallization.
4.Product polishing: packaging of product.
Additional Information:
Recombinant DNA technology consists of steps such as:
1.Isolation of genetic material.
2.Cutting of DNA at specific sites with the help of restriction enzymes.
3.Extraction and amplification of genes of interest.
4.Insertion of recombinant DNA in the host cell.
5.Downstream processing.
Note:
Downstream streaming is the last part of recombinant DNA technology which includes purification and separation of the desired product.
Recently Updated Pages
Basicity of sulphurous acid and sulphuric acid are
What is the stopping potential when the metal with class 12 physics JEE_Main
The momentum of a photon is 2 times 10 16gm cmsec Its class 12 physics JEE_Main
Using the following information to help you answer class 12 chemistry CBSE
Which of the following would not be a valid reason class 11 biology CBSE
Why should electric field lines never cross each other class 12 physics CBSE
Trending doubts
Difference Between Plant Cell and Animal Cell
Difference between Prokaryotic cell and Eukaryotic class 11 biology CBSE
How do you solve x2 11x + 28 0 using the quadratic class 10 maths CBSE
Select the correct plural noun from the given singular class 10 english CBSE
What organs are located on the left side of your body class 11 biology CBSE
The sum of three consecutive multiples of 11 is 363 class 7 maths CBSE
What is the z value for a 90 95 and 99 percent confidence class 11 maths CBSE
Fill the blanks with the suitable prepositions 1 The class 9 english CBSE
How many squares are there in a chess board A 1296 class 11 maths CBSE