Question

The thermostable enzymes, Taq and Pfu, isolated from thermophilic bacteria are
A. RNA polymerases
B. DNA Ligases
C. DNA polymerases
D. Restriction endonucleases

Answer 1

Hint: 'Taq’ and ‘Pfu’ are both thermally stable enzymes used in polymer chain reactions. They are isolated from specific thermophilic bacteria.
They're used for polymerization, which is widely utilized in the polymerase chain reaction.

Complete answer: Pfu DNA polymerase is an enzyme found within the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to repeat the organism's DNA, during cellular division.
Within the laboratory settings, Pfu is employed to amplify DNA within the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a replacement strand of DNA, during each extension step.
Taq polymerase is essentially a thermostable DNA polymerase I, named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it had been originally isolated.
Its name is usually abbreviated to Taq or Taq pol. It gets utilized very often in the polymerase chain reaction (PCR), which is a method for greatly amplifying the number of short segments of DNA.

So, the correct answer is option A. DNA polymerases.

Note: Scientific research led by Eric Mathur at the biotech company Stratagene, based in La Jolla, California, discovered Pfu DNA polymerase which exhibits significantly higher fidelity of replication than Taq DNA polymerase in the year 1991.