Question

The stain used to visualize mitochondria is
A) Fast green
B) Safranin
C)Acetocarmine
D) Janus green

Answer 1

Hint: Mitochondria is the organelle in which various biochemical reactions occur like respiration and ATP(energy) formation. Staining is a process in which various types of stain are used to increase the visualization.

Complete answer:
In the mitochondria electron transfer chain is occurring. It is the process by which energy production occurs in our body through the Oxidation and reduction.
Oxidation and reduction is a key point of interest.
Any alteration in Oxidation and reduction process causes the changes in the color of stain according to the oxygen present in the mitochondria and helps in visualization.
Mitochondria visualization is important in various studies like physiology, pathology and pharmacology.
Janus green B is the stain that interacts with DNA and used in various histology studies.

Additional information:
When mitochondria comes in contact with the stain, then it absorbs all parts at the starting and the mitochondria is reduced to the non mitochondria portion of the cell.
Any dehydrogenase systems reduced the Janus green if that system had the capacity to form the reduced flavoprotein.

Hence the correct answer is the Janus green B.

Note:After the reaction with mitochondria Janus green shows two colors:-in presence of oxygen indicator oxidize to blue color and in absence of oxygen Janus green reduces to pink color.