Question

The organic compound present in traces in living cells can be detected and isolate by
a. Centrifugation
b. Tracer technique
c. Chromatography
d. Microscopy

Answer 1

Hint: The chemical compound which is present in living things are called organic compounds hence they are carbon-containing compounds and associated with organisms. There are various types of an organic compound in the living things but majorly it is divided into four categories – carbohydrate, protein, nucleic acid and lipids.

Complete answer:
> Option A: Centrifugation-
It is a technique for the separation of particles from the solution depending upon the density, viscosity, size and shape of the medium.

> Option B: Chromatography-
It is an analytical technique, it is used for the separation of a mixture of a chemical compound into its component. It is done so that the components are analyzed thoroughly.

> Option C: Tracer technique-
This technique is used in the biosynthesis pathway in plants to find out or trace out the various intermediates and steps in the biogenic experiments. In this technique, isotopes are used most prefer radioactive isotopes. These isotopes are incorporated into the presumed precursor of the plant’s metabolites and used as markers.

> Option D: Microscopy-
It is a technical field where microscopes are used to observe the object such as living cells which are seen with the naked eyes.

Hence, the correct answer is option (C).

Additional information:
The principle of chromatography technique is based on different interaction of components with two phases (mobile phase and stationary phase) as the components travel through the supporting medium. The compounds are separated and distributed between these phases.
- Mobile phase: solvent that flows through the supporting medium.
- Stationary phases: layer on the supporting medium that interacts with the analytes.
- Supporting medium: The stationary phase is bound on the solid surface.

Note: Chromatography can have different types such as gas chromatography, liquid chromatography, ion-exchange chromatography, and affinity chromatography. The chromatography techniques worked on the same principles.