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The best and latest technique for isolation, purification, and separation of organic compounds is:
A. Crystallisation
B. Distillation
C. Sublimation
D. Chromatography

Answer
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Hint: By best technique we mean the maximum efficiency. The purpose for which each of the mentioned methods are used, is a way of choosing the best out of them.

Complete step-by-step answer:
Chromatography is the most useful and latest technique of separation and purification of organic compounds. It was first used for the purpose of separating a mixture of coloured substances.
Therefore, the best and latest technique for isolation, purification, and separation of organic compounds is chromatography.
So, the correct answer is Option D.

In chromatography there is separation of different parts of chemical mixtures onto an adsorbent material that can be individually analyzed because different parts are caught on the material at different rates.

Additional Information:
Crystallization is the process by which a solid forms where the atoms or molecules are highly organized into a structure known as a crystal. Some of the ways by which crystals form are precipitating from a solution, freezing or more rarely deposition directly from a gas.
Distillation is the process of separating the components of substances from a liquid mixture by using selective boiling and condensation distillation method, this results in essentially complete separation or it may be a partial separation that increases the concentration of selected components in the mixture.
Sublimation is a transition of a substance directly from the solid to the gas state without passing through the liquid state

Note: Chromatography is defined to be a process of separating components of a mixture. To get the process started, the mixture is dissolved in a substance, called the mobile phase, which carried it through a second substance, called the stationary phase.