Question

The basic procedure involved in the synthesis of a recombinant DNA molecule is depicted below. The mistake in the procedure is.
A. Enzyme polymerase is not included.
B. The mammalian DNA is shown double-stranded
C. Two different restriction enzymes are used.
D. only one fragment is inserted.

Answer 1

Hint: The recombinant DNA molecule is the DNA molecules that are formed in the laboratory by genetic recombination like molecular cloning, the recombinant DNA formed from multiple sources and creates a new sequence that is not present naturally but created artificially by scientists.

Complete answer:
The recombinant DNA molecule is made up of two different fragments of DNA that come from different sources and recombinant DNA is also called chimeric DNA and recombinant DNA technology used the palindromic sequence which produces the blunt and sticky ends.
To cut the DNA strand at the specific site and paste with other strands that come from a different source (cell) and need different sets of enzymes, restriction enzyme used to cut the DNA away from the palindromic site and between the same two bases in opposite strands, which leave the single-stranded unpaired base at the ends, that act as cohesive or sticky ends. Such cohesive ends facilitate the action of enzyme DNA ligase and the same enzyme used in the formation of sticky ends that is further used to combine different DNA segments and form recombinant DNA molecules.
In recombinant DNA same restriction enzyme used to identify the same sequence in both different DNA segment and cut at the same site, which is pasted by DNA ligase enzyme, if two different enzymes are used then they cut the different DNA segment at a different site, which may not able to paste by DNA ligase enzyme and not form recombinant DNA molecule.
Hence, the correct option is C.

Note: The recombinant DNA technology is vastly used in biotechnology, medicine, and research. recombinant DNA technique used to create a different genetic organism that is used in the agriculture sector either crop or animal agriculture sector.