Question

Temporary mount of a peel is made in
(a) Alcohol
(b) Water
(c) Glycerine
(d) Acetone

Answer 1

Hint: We use this substance at home as a moisturizer to cure or prevent dryness, roughness, scaly, and itchy skin, also to treat minor skin irritations caused due to diaper rash, skin burns from radiation therapy.

Complete answer:
Glycerine is used to make the temporary mount of a peel. Glycerine is used for this purpose because it is a perfect dehydrating agent and prevents the specimen from drying out. Another reason for using glycerine for the temporary mount is that it is highly refractive in nature and it tends to reflect the light better as a result, the specimen can be clearly observed under the microscope.
-Temporary mount is also known as a wet mount in which the specimen is placed in a drop of water or any liquid held between the slide and the coverslip by surface tension.
-To view the particles of stomata easily during the experiment glycerine is used which keeps the stomata wet and moisturized.
-Glycerine used in temporary mount does not make the cells swell or shrink, it will remain in their natural form for our study.
-Glycerine forms a protective layer when applied over the specimen to keep it moist. Since it is a dehydrating agent it avoids drying of the specimen.
-The temporary mount is prepared quickly since specimen fixation, dehydration and staining are not compulsory, so students can easily learn how to mount a slide.
-The specimen will not be stained properly because the stain will be washed off with glycerine if staining is done after adding glycerine.

So, the correct answer is, 'Glycerine'.

Note:
-Glycerine is used for this purpose because it is a perfect dehydrating agent and prevents the specimen from drying out.
-Glycerine forms a protective layer when applied over the specimen to keep it moist. Since it is a dehydrating agent it avoids drying of the specimen.
-Another reason for using glycerine for the temporary mount is that it is highly refractive in nature, so reflects light in a better way to make the cells more clearly visible under a microscope.