Question

Stirred-tank bioreactors have been designed for
A. Availability of oxygen throughout the process
B. Addition of preservation to the product
C. Purification of the product
D. Ensuring anaerobic conditions in the culture vessel

Answer 1

Hint: Stirred tank bioreactors allow for limited sample volumes to be taken to test and use the temperature and pH-controlled control system.

Complete Answer:
- A reactor with a stirred tank is normally cylindrical or curved to make the reactor material mixer simpler. The stirrer system even makes it easier for the bioreactor to mix and to provide oxygen. Air may also be bubbled by the reactor. The stirred-tank bioreactor is one of the most standard bioreactors.
- The agitator or impeller which carries out a broad range of functions is the core component of the agitating tank bioreactor: heat and mass transfer, aeration and homogenization mixing. The bioreactor has an agitator system, an oxygen supply system and a foam control system, a temperature control system, a pH controller and sampling port, in order to extract small culture volumes on a regular basis.
- The stirred tank bioreactors are typically cylindrical to allow mixing the reaction contents or with a curved foundation. The Stirrer makes the whole bioreactor much easier to combine and to provide oxygen. These bioreactors ensure the supply of oxygen during a process. The reactor may also bubble air alternatively.
- The most commonly used bioreactors for GMP producing monoclonal antibody and other biological uses in the animal cell culture are therefore the stirred tanks in the current pharmaceutical industry.

Thus, the correct answer is option A. i.e., Availability of oxygen throughout the process.

Note: Generally, the stirred tank bioreactor has various advantages for the cultivation of shear-sensitive cells: established industrial ability, proven operating efficiency, ease of homogeneous conditions and facility for scale-up and control.