Question

Mast cells contain vesicles that store large amounts of histamine. After staining with eosin, these vesicles are stained red in colour. Identify which of the following interactions is involved between histamine and eosin?
A. Hydrophobic interaction
B. Electrostatic interaction
C. Covalent bonding
D. Hydrogen bonding

Answer 1

Hint: A mast cell (also referred to as a labrocyte ) could be a migrant cell of animal tissue that contains several granules made in amino alkane and anticoagulant medication. Specifically, it is a sort of white corpuscle derived from the myeloid vegetative cell that's a neighbourhood of the immune and neuroimmune systems. Mast cells were discovered by bacteriologists in 1877.

Complete answer:
Eosin is the most typical dye to stain the living substance in microscopic anatomy. It's associates degree acidic dye that binds to basic elements of a cell, principally proteins situated within the living substance. It offers a bright pink colour that contrasts that navy nuclear haematoxylin staining essential amino acid, histidine, an essential amino acid (at each acidic and neutral pH) and tryptophan (at acidic pH) residues of a supermolecule bind electrostatically to group and phenoplast teams of the dye to provide a stable soluble protein-dye advanced. As amino alkane is supermolecule thus fluorescent dye and amino alkane bind thanks to electricity interaction and provides the red colour.
So, the proper answer is “Electrostatic interaction”

Hence B is the correct option.

Note:
Mast cell, tissue cell of the system of vertebrate animals. Mast cells mediate inflammatory responses like hypersensitivity and allergies. they're scattered throughout the connective tissues of the body, particularly at a lower place the surface of the skin, close to blood vessels and bodily fluid vessels, among nerves, throughout the system respiratory, and within the biological process and urinary tracts. Mast cells store a variety of various chemical mediators—including amino alkane, interleukins, proteoglycans (e.g., heparin), and varied enzymes.