Question

In anther culture some diploid plants were reported with haploid they are evolved from.
A) prothallial cell of pollen grain
B) generative cell of pollen grain
C) cell of anther wall
D) exine of pollen grain

Answer 1

Hint: Anther culture means plant regeneration from the haploid microspore cells with the aim of haploid and dihaploid plant production. It allows successful breeding of crips and is also an efficient way to create inbred line development.

Complete answer:
Another culture is used to develop instant homozygous inbred lines, avoiding the lengthy time needed using traditional selfing methods including bud pollination. Anther culture results in haploid plants, easily identified by their smaller sterile flowers. 89 Chromosome numbers are doubled using colchicine to give doubled haploids.

Spontaneous diploids can also occur. Depending on incompatibility status, doubled haploids can be used as parents for hybrids or as ‘cultivars’ in their own right. The technique is successful in Calabrese. The regenerants were all diploid but their origin was unclear. The authors did not exclude the chromosome duplication in plant regenerants. In the experiment of Kong et al.

Regenerants were obtained in all six cultivars used. The adopted plants grew well but did not set the seeds. Unfortunately, the number of chromosomes was not counted in this case. Haploid plants are of special interest to the geneticist and plant breeder because by chromosome doubling completely homozygous diploid plants can be obtained from haploid plants. Owing to the great theoretical and applied value of haploids, many methods have been tried to induce their occurrence.

Therefore, the correct answer is option (C) cell of anther wall.

Note: Various techniques of artificial haploid production, such as treatment with irradiated pollen, delayed pollination, utilization of alien cytoplasm or pollinator, distant hybridization, polyembryony, twin seedlings and physical and chemical treatments, have been employed.