Question

Identify correct combinations.

List i	List ii	List iii
A) PCR	i.Specific base sequence	Taq polymerase
B) Downstream processing	ii. Inheritable gene	Il Ampicillin
C) Restriction endonuclease	iii. Amplification of DNA	EcoRI
D) Selectable marker	iv Transformation and non-transformationV. Product separation and purification	IV Quality control V. Ori

A. A-iii- I, B-v-IV,C-ii-V. D-i-III.
B. A-ii-IV, B-iv-I, C-ii-V. D-iii-I
C. A- iii- I, B- v- IV, C-i-III,D-iv- II
D. A-iii-I, B-iv-I,C-i-III, D-ii-IV

Answer 1

Hint: PCR stands for a polymerase chain reaction, while downstream processing is the purification of biosynthetic materials and restriction endonucleases is the type of enzyme used for many purposes, and selectable markers are used for artificial selection.

Complete answer:
Polymerase chain reaction( PCR ): It is the method, widely used to rapidly make millions to billions of copies of specific DNA samples.
And this method allows scientists to take a very small sample of the DNA and amplify it to large enough.
It depends on Thermocycler.
Enzymes used for this process are DNA polymerase enzyme and Taq polymerase.
Downstream processing: It refers to the recovery and purification of biosynthetic products, and mainly pharmaceuticals.
It also purifies the natural sources like plant and animal tissues.
It includes the recycling of salvageable components.
The stages include, removable instabilities, product isolation, product purification, and product polishing. So it is mainly the concentration and quality of the product.
Restriction endonuclease: This is the group of enzymes, which target the DNA at the specific site and are useful to divide into fragments.
They are of again 4 types, based on composition, coenzyme factor, Nature of target sequence, and the position of DNA cleavage.
Selectable markers: It is the gene, a specific DNA sequence, which is introduced into the cell, it may be into bacterium, or to a cell culture, that mainly confers the trait suitable for artificial culture.
Examples include beta lactamase confers Ampicillin.
E Coli confers Triclosan.

Correct option: A) A-iii-I, B-v-IV, C-ii-V, D-i-III.

Note:
The above given procedures are all genetic engineering and biotechnology procedures, where some are used for DNA amplification, and some are used for artificial culture and one is used for fragmentation of the DNA molecule.