Question

A three-dimensional image of the object can be produced using
A. Compound microscope
B. Dark-field microscope
C. Transmission electron microscope
D. Scanning electron microscope

Answer 1

Hint:-There are numerous sizes and special applications for microscopes. The electron microscope and light microscope are the most popular kinds of microscopes. The key distinction between the light microscope and the electron microscope (getting a large image resolution) is that electron beams are used to magnify an object's image, while visible light is used to magnify images of tiny areas of materials or biological specimens in the light microscope.

Complete answer:
Various types of microscopes are discussed below:
Compound microscope:- A compound microscope is an elaborate set of a range of lenses that gives a highly maximized and magnified picture of living microscopic entities and other complex information or cells and tissues. As the picture is created in the center of a brightly illuminated field, the popular light microscope is often called a bright-field microscope.
Dark-field microscope:- In order to illuminate unstained samples, dark-field microscopy is preferably used to make them appear brightly illuminated against a dark backdrop. When there is no sample on the microscope platform, the entire field appears dark; when a sample is on the platform, the rays are scattered by the sample and thus captured in the objective lens, and make the image. It enables live bacteria to be visualized and distinguishes some shape (rods, curved rods, spirals, or cocci) and motion.
Transmission electron microscope:- A method used to observe the characteristics of very tiny specimens is transmission electron microscopy (TEM). To allow a scientist to study characteristics such as structure and appearance, the technology makes use of an accelerated beam of electrons that passes through a very thin piece of the specimen. TEM demands very thin specimens that are electron-semi-transparent.
Scanning electron microscope:- The scanning electron microscope (SEM) is a type of electron microscope which, by scanning with a high-energy electron beam, produces images of an object. The electron beam's behavior stimulates the emission from the surface of the specimen of high-energy back-scattered electrons and low-energy secondary electrons. A beam of electrons travels back and forth over the surface of a cell or tissue during scanning electron microscopy (SEM), producing a clear picture of the 3D surface.
Thus option (D), ‘scanning electron microscope’ is the correct answer.

Note:-
Applications of SEM include the following:
- Characterization of metals, glasses, ceramics, and polymers with surface defects, stains, and residues
- Monitoring the effect of acid degradation on human tooth enamel.
- Micro-detail measurement on wear scars and engineered metal surfaces
- Measurement of the effects of therapies using replication methods on human skin texture.