
A piece of nucleic acid used to find a gene by forming a hybrid with it, is called as
A. Vector
B. Probe
C. Retrovirus
D. Restriction sequence
Answer
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Hint: Nucleic acids are the substances that constitute the genetic material of the person which include the DNA and RNA, where they are made up of nucleotides, which are of 2 varieties called as purines and pyrimidines and they are complementary to each other.
Complete answer: A piece of nucleic acid to form the hybrid is generally used in the hybridization technique, where DNA are double stranded structure where both strands are complementary to each other, whereas in hybridization strands are separated where it generally not possible in general conditions but only in some special conditions during annealing this strands or a part of the strands are separated with each other, so it is complementary to any surrounding strands.
Where lowering the temperature of the surrounding molecule helps to make the strand or part or sequence to anneal or to hybridize it.
Hybridization of nucleic acids are generally applied in some cases such as to see the genetic relatedness of the species by doing the annealing of the nucleic acids of the both species.
In case of closely related species mostly the sequences of the bases are similar and the higher temperature is required to make the sequence possible for hybridization, so DNA probe is the correct option.
When coming to other options, vector is also called as plasmid which is mainly used to produce the recombinant DNA, and the retrovirus is a one of the type of virus, and Restricted sequences are not used for hybridization, so these options are wrong.
So, the correct option is B.
Note: A perfect nucleic acid hybridization assays generally involve use of DNA probe or any nucleic acid probe where to identify related DNA or RNA molecules where mostly it is possible only with sequence having high degree similarity, in the given mixture of molecules.
Complete answer: A piece of nucleic acid to form the hybrid is generally used in the hybridization technique, where DNA are double stranded structure where both strands are complementary to each other, whereas in hybridization strands are separated where it generally not possible in general conditions but only in some special conditions during annealing this strands or a part of the strands are separated with each other, so it is complementary to any surrounding strands.
Where lowering the temperature of the surrounding molecule helps to make the strand or part or sequence to anneal or to hybridize it.
Hybridization of nucleic acids are generally applied in some cases such as to see the genetic relatedness of the species by doing the annealing of the nucleic acids of the both species.
In case of closely related species mostly the sequences of the bases are similar and the higher temperature is required to make the sequence possible for hybridization, so DNA probe is the correct option.
When coming to other options, vector is also called as plasmid which is mainly used to produce the recombinant DNA, and the retrovirus is a one of the type of virus, and Restricted sequences are not used for hybridization, so these options are wrong.
So, the correct option is B.
Note: A perfect nucleic acid hybridization assays generally involve use of DNA probe or any nucleic acid probe where to identify related DNA or RNA molecules where mostly it is possible only with sequence having high degree similarity, in the given mixture of molecules.
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