Question

Which of the following factors does not affect an enzyme activity?
A.pH levels
B.Inhibitors
C Temperature
D.Enzyme cofactors
E.Enzyme concentration

Answer 1

Hint: We are asked about the factors which affect the enzyme activity. We will check how the enzyme activity is dependent on the given options. The option which does not affect the enzyme activity will be the answer.

Complete step by step solution: The first option given is pH level. Every enzyme has a specific enzyme for its activity. This range of pH is known as optimum pH. When the pH is increased above or below this level then the enzyme activity will get reduced considerably and the enzyme activity will get reduced.
The second option is inhibitors. Inhibitors are substances which alter the chemical activity of the enzyme. So when inhibitors are used the enzyme activity will reduce.
The third option is temperature. On increasing the temperature the enzyme activity increases and on lowering the temperature and enzyme activity reduces. But the temperature should not be very high as high temperature will cause denaturation of enzyme i.e. enzyme shape will be damaged and enzyme activity will stop.
The fourth option is enzyme cofactors. Cofactors are metals or small organic substances and their primary function is to assist the reaction which enzymes cannot perform alone. The increase the efficiency of the enzyme. So in the presence of cofactors the enzyme activity increases.
The fifth option is enzyme concentration. This enzyme concentration increases the enzyme activity but only when there is presence of substrate. But once the substrate is completed there will not be any substrate for the enzyme to bind. So this will not affect the enzyme activity. So this will be the answer.

The correct option is E. enzyme concentration.

Note: For solving this type of question it should be known that how each of the factors given in the option affect enzyme activity. It should be noted that increasing the temperature activity only increases only to a certain limit.