
Thin layer chromatography is:
A.Partition chromatography
B.Electrical mobility of ionic species
C.Adsorption chromatography
D.None of the above
Answer
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Hint: Thin layer chromatography is based on the relative value of the compounds. We very often use thin layer chromatography in laboratories. The spots are made visible by UV light.
Complete step by step answer:
Chromatography is used in two separate non volatile mixtures. Adsorption is the process in which one molecule known as adsorbate gets absorbed on the other molecule that is known as adsorbent. The term adsorbate and adsorbent are analogous to solute and solvent in a solution.
Chromatography takes place on a sheet of aluminium foil or plastic on which a thin layer of adsorbent material is placed. The adsorbent material mainly used is Aluminium oxide, cellulose or silica gel because they are good adsorbent. It is based on the relative affinity of the compounds. There are two phases in a chromatography, one is the mobile phase and the other one is stationary phase. Mobile phase moves over the stationary phase. If a material has more affinity towards stationary phase then it will move slower and the one that is high affinity towards mobile phase will move faster. So, this will create a separation between the mixtures. We will get separate spots for the separate components in a mixture.Hence thin layer chromatography is a type of adsorption chromatography
Partition chromatography is the method to separate the components between two liquid phases using a column. Electrical mobility of ionic species is defined as the movement of ions towards the respective electrode when electricity is applied to them.
Hence, the correct option is C.
Note:
Thin layer chromatography is used in cosmetic industries, it is used to separate and identify colours, sweetening agents, preservatives in the food industry. It is also used in separating multicomponent pharmaceutical products. It is also used in biochemical analysis and to identify natural products like essential oils and waxes.
Complete step by step answer:
Chromatography is used in two separate non volatile mixtures. Adsorption is the process in which one molecule known as adsorbate gets absorbed on the other molecule that is known as adsorbent. The term adsorbate and adsorbent are analogous to solute and solvent in a solution.
Chromatography takes place on a sheet of aluminium foil or plastic on which a thin layer of adsorbent material is placed. The adsorbent material mainly used is Aluminium oxide, cellulose or silica gel because they are good adsorbent. It is based on the relative affinity of the compounds. There are two phases in a chromatography, one is the mobile phase and the other one is stationary phase. Mobile phase moves over the stationary phase. If a material has more affinity towards stationary phase then it will move slower and the one that is high affinity towards mobile phase will move faster. So, this will create a separation between the mixtures. We will get separate spots for the separate components in a mixture.Hence thin layer chromatography is a type of adsorption chromatography
Partition chromatography is the method to separate the components between two liquid phases using a column. Electrical mobility of ionic species is defined as the movement of ions towards the respective electrode when electricity is applied to them.
Hence, the correct option is C.
Note:
Thin layer chromatography is used in cosmetic industries, it is used to separate and identify colours, sweetening agents, preservatives in the food industry. It is also used in separating multicomponent pharmaceutical products. It is also used in biochemical analysis and to identify natural products like essential oils and waxes.
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