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Last updated date: 03rd Dec 2023
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MVSAT Dec 2023

The technique of developing pure culture of bacteria was developed by
A. J. Lister
B. L. Pasteur
C. R. Koch
D. A.V. Leeuwenhoek

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Hint: A pure culture is a culture which is derived from a mixed culture. It contains one type of cell which is derived from the single cell. All the cells are the clones of the parent cell. The pure culture is obtained in the laboratory by using agar gel media.

Complete answer –
We already know that the pure culture is obtained by transferring a small sample in a new and sterile growth media. It is done in a way that the individual cells are dispersed across the surface of the media. It can also be done by thinning the sample before inoculating in the new media.
Let us discuss the contributions of these pioneer scientists.
Joseph Lister was the pioneer of antiseptic surgery. He discovered penicillin.
L-Pasteur- He discovered the process of vaccination, pasteurization and microbial fermentation.
R-Koch- He was a German scientist who discovered the causative agents for deadly diseases like Tb, cholera, anthrax. He discovered solid culture media and was the first to obtain pure culture of bacteria.
A.V Leeuwenhoek- He is known as the father of microbiology. He made a single lens microscope which helped him to visualize protozoa, bacteria.

The correct answer is C)R. Koch.

Note –
The pure culture helps in isolation of the pure strain of the microorganism. This helps in studying the characteristics of the causative agent. Pure culture has a single type of colony whereas a mixed culture shows various colonies of different colours.