Question

The technique involving the isolation and fusion of protoplasts and culturing the hybrid protoplast to regenerate the whole plant is known as
A. Para sexual hybridization
B. Genetic engineering
C. Protoplast culture
D. Androgenic haploids

Answer 1

Hint: To regenerate new plant, culturing of protoplast is done.
The steps involve isolation and fusion of protoplasts and then culturing the hybrid one.

Complete answer: A protoplast refers to the entire plant cell without its cell wall, just surrounded by a plasma membrane. Though it is quite fragile in nature if cultured, it can grow into a complete whole plant.
To do this, protoplast is first separated from plant tissue, and then they are fused.
After that hybrids are cultured to produce a new whole plant.

Additional information: Functions OF Protoplast culture include:
(1) Providing the protoplast, or living cell, with mechanical protection and a chemically buffered surrounding,
(2) Providing a porous medium for the circulation and distribution of water, minerals, and other small nutrient components.

So, the correct option is C. Protoplast culture

Note: 1. Para sexual hybridization: It is a hybridization technique in which protoplast fusion occurs and plants are regenerated from the resulting fusion products.
2. Genetic engineering: It is the method of manipulating the genetics of an organism.
3. Androgenic haploids: When haploid plants are produced by culture of anthers/pollen, they are known as androgenic haploids.