Question

The stain used to visualise mitochondria is
A. Fast Green
B. Safranin
C. Acetocarmine
D. Janus Green

Answer 1

Hint: The semi-autonomous and double-membrane-bound organelle that is found in most eukaryotic organisms is called Mitochondria. Some cells in some multicellular organisms may. However, it lacks mitochondria. The mitochondrion has some most prominent roles to produce the energy currency of the cell, ATP that is of phosphorylation of ADP, through respiration, and to regulate cellular metabolism.

Complete answer:
-Fast green: This is a green dye that is used in optical microscopy that stains cellulose, cytoplasm, collagen, and mucus green. And to stain plant tissues it is frequently used, with safranin as a counterstain. Unlike light green, a similar dye, and also it does not fade easily.
So, option (A) is incorrect.
-Safranin: Biological stain used in histology and cytology is called Safranin. In some staining protocols safranin is used as a counterstain, coloring cell nuclei red. And this is the classic counterstain in both Gram stains and endospore staining.
So, option (A) is incorrect.
-Acetocarmine: Acetocarmine is such a strain used to stain nucleic acid inside cells. As acetocarmine specifically-strain chromosomes apart from the cytoplasm, it can be used to visualize chromosomes in mitotic studies.
So, option (C) is incorrect.
-Janus Green: The Janus Green is a dark green/dark brown/dark black powder. Janus Green should be oxidized to become colored. In this way, this dye can be used to specifically stain mitochondria in living cells. Stain biomolecules, nucleic acids, and chromosomes use it. Janus Green B acts as an antimalarial agent. And also diagnostic assays and diagnosis of disease related to amyloid accumulation facilitated by Janus green.
So, the correct answer is “Option D”.

Note:
Each mitochondrion is a double membrane-bone structure with outer and inner membranes. The space between the outer and inner membrane is called intermembrane space. The outer membrane is fairly smooth. But the thing is that the inner membrane is highly convoluted, forming folds called cristae. The inner membrane is also very impermeable to many salutes due to the very high content of a phospholipid called cardiolipin. The inner membrane's surface area is greatly increased by cristae. The two faces of this membrane are referred to as the matrix side (N-inside) and the cytosolic side (P-side). The inner membrane contains an enzyme complex called ATP synthase that makes ATP. The passage of small molecules across the mitochondrial outer membrane is allowed by the mitochondria porins or voltage-dependent anion-selective channels.