Question

The polymerase chain reaction is a technique that:
A. Is used for in vivo replication of DNA
B. Is used for in vivo synthesis of mRNA
C. Is used for in vitro synthesis of mRNA
D. Is used for in vitro replication of specific DNA sequence 1 NA 1.

Answer 1

Hint: The full form of the abbreviation PCR is Polymerase chain reaction. One can say that the Polymerase chain reaction is the technique which needs a test tube to complete the procedure. This method completes the process in a test tube rather than an organism. One can make the use of the process of the Polymerase chain reaction make millions to billions of copies of a specific DNA sample in a short period of time.

Complete answer:
For sake of the process of amplification of DNA fragments, we use the technique of Polymerase chain reaction. There are three steps which are included in the process of amplification cycle. We can say that the procedures of denaturation, annealing and polymerization are the three steps of the amplification cycle. These steps are made to repeat for ‘n’ times.
The transfection of cells with wanted mRNAs can induce the expression of target proteins by completing the process of vitro synthesis of modified mRNA.
The transcription is the process of obtaining the mRNA from DNA by the procedure of vivo synthesis.
By nock translation, vitro replication of specific DNA sequence 1 NA 1 is done. It helps in enabling the enzyme to change nucleotides in the developing strand of DNA.
So, the correct answer is “Option A”.

Note:
Polymerase chain reaction is a method widely used by the various scientists. This procedure helps the scientists to take a very trivial part of the sample of one’s DNA and make their best to intensify it to a large enough quantity to make a study on it in detail. This process collects the small pieces of the DNA and amplifies it to make million to billion copies of the same DNA. Thus, this process is very helpful to the scientist.