Question

Principle of centrifugation is:
A. Denser particles stay at the top and lighter particles are forced to the bottom
B. Lighter particles stay at the top and the denser particles are forced to the bottom
C. High speed rotation increases the velocity of the particles and make then charged
D. Particles become weightless due to high speed rotation

Answer 1

Hint: There are two types of forces that are going to act on the object which is in rotation. They are centripetal force and centrifugal forces. The centripetal forces make the object to pull towards the center and centrifugal force makes the object to move away from the center.

Complete answer:
- In the question it is asked to find the principle of the centrifugation among the given options.
- Centrifugation is a technique used in various purposes to separate heavier particles from lighter particles.
- If the centrifugal force is going to act on the heavier and light particles, then the heavier particles are going to get settled and the lighter particles are going to occupy the surface of the solution which we are going to apply.
- Means the heavier particles are going to occupy the bottom and the lighter particles stay at the top.
- Therefore, the principle of centrifugation is Lighter particles stay at the top and the denser particles are forced to the bottom.

So, the correct option is B.

Note:
In laboratories at the time of test blood for different purposes centrifugation is going to apply and by this forces the heavier particles like RBC (Red blood cells) are going to occupy the bottom and the plasma which is lighter stays at the top.