
In reverse phase chromatography, the stationary phase is made:
(A) Non-polar
(B) Polar
(C) Either polar or nonpolar
(D) None of the above
Answer
476.1k+ views
Hint: In reverse phase chromatography, the polarity of the phases is inverted as compared to the normal phase chromatography. In reverse phase chromatography, silica cannot be used as a stationary phase.
Complete step by step solution:In a broad sense, chromatography can be divided into two categories depending upon the phase system, normal phase chromatography and reverse-phase chromatography.
- We know that chromatography is a technique to separate the solute components from a mixture.
- We use two phases to separate the solutes of the mixture. The names of the phases are the mobile phase and a stationary phase.
- Mobile phase is a phase which is not stationary and it moves through the stationary phase. The stationary phase is a phase which generally does not move and a mobile phase is passed through it.
- In reverse phase chromatography, the stationary phase is a non-polar compound and mobile phase is a polar compound.
- So, a non-polar stationary phase has more affinity towards the non-polar solutes and polar mobile phase has more affinity towards polar solute molecules.
- Thus, we can say that as we pour the mobile phase, the solutes will interact with both the stationary phase and mobile phase. Depending upon the nature of the solute, it will have a slow or fast speed of movement along with the flow.
- If the solute is polar, then it will move faster as the mobile phase is also polar and if the solute is non-polar, then it will move slower as the stationary phase is nonpolar.
- Thus, we can say that in reverse phase chromatography, the stationary phase is non-polar.
Therefore, the correct answer is (A).
Note: Do not get confused between the polarity of phases in normal phase and reverse phase chromatography. In normal phase chromatography, the stationary phase is polar and the mobile phase is non-polar. The phases are inverted in reverse phase chromatography.
Complete step by step solution:In a broad sense, chromatography can be divided into two categories depending upon the phase system, normal phase chromatography and reverse-phase chromatography.
- We know that chromatography is a technique to separate the solute components from a mixture.
- We use two phases to separate the solutes of the mixture. The names of the phases are the mobile phase and a stationary phase.
- Mobile phase is a phase which is not stationary and it moves through the stationary phase. The stationary phase is a phase which generally does not move and a mobile phase is passed through it.
- In reverse phase chromatography, the stationary phase is a non-polar compound and mobile phase is a polar compound.
- So, a non-polar stationary phase has more affinity towards the non-polar solutes and polar mobile phase has more affinity towards polar solute molecules.
- Thus, we can say that as we pour the mobile phase, the solutes will interact with both the stationary phase and mobile phase. Depending upon the nature of the solute, it will have a slow or fast speed of movement along with the flow.
- If the solute is polar, then it will move faster as the mobile phase is also polar and if the solute is non-polar, then it will move slower as the stationary phase is nonpolar.
- Thus, we can say that in reverse phase chromatography, the stationary phase is non-polar.
Therefore, the correct answer is (A).
Note: Do not get confused between the polarity of phases in normal phase and reverse phase chromatography. In normal phase chromatography, the stationary phase is polar and the mobile phase is non-polar. The phases are inverted in reverse phase chromatography.
Recently Updated Pages
Master Class 11 Economics: Engaging Questions & Answers for Success

Master Class 11 Business Studies: Engaging Questions & Answers for Success

Master Class 11 Accountancy: Engaging Questions & Answers for Success

The correct geometry and hybridization for XeF4 are class 11 chemistry CBSE

Water softening by Clarks process uses ACalcium bicarbonate class 11 chemistry CBSE

With reference to graphite and diamond which of the class 11 chemistry CBSE

Trending doubts
10 examples of friction in our daily life

One Metric ton is equal to kg A 10000 B 1000 C 100 class 11 physics CBSE

Difference Between Prokaryotic Cells and Eukaryotic Cells

State and prove Bernoullis theorem class 11 physics CBSE

What organs are located on the left side of your body class 11 biology CBSE

How many valence electrons does nitrogen have class 11 chemistry CBSE
