Question

Fungi can be stained by
(A) Safranin
(B) iodine
(C) Lactophenol
(D) Cotton blue

Answer 1

Hint: Staining is an additional technique used to increase contrast in the microscopic picture in microscopy. In biology and medicine, stains and dyes are many times used to show constructions in organic tissues for viewing, frequently with the help of a variety of microscopes. Lactobacillus belongs to the community of bacteria that ferment and, in the absence of oxygen, flip the sugars into lactic acid.

Complete answer:
Now let us find solution from given options:
(A) Safranine : The safranin stain is usually observed to be a cheaper and safer- lab stain. Safranin is a mostly used stain for chromosomes. It can be utilised for staining animals as well as plant cells for better cytological and histological analysis.
(B) iodine : iodine is a stain that is used for staining gram negative and gram positive bacteria. This is a primary stain used in the staining techniques.
(C) Lactophenol : Lactophenol is a stain that is utilised to distinguish fungal mycelia from plant cells, which stains fungal hyphae in blue colour, leaving the plant cell wall unstained.
(D) Cotton blue : Cotton blue is defined as an acid dye that stains the chitin present in the cell walls of fungi. So this is used to stain fungi.

Hence the correct answer is option ‘D’.

Note: Lactophenol cotton blue is a combination of methyl blue, a histological stain, and lactophenol. It is used in wet-mount preparations for visualization of fungal structures, especially in scientific mycology.