
What is the function of restriction enzymes in nature?
Answer
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Hint: Restriction enzymes (also known as RE) have the appearance of scissors, which matches their name of ‘molecular scissors' Microorganisms and archaea produce these compounds to protect themselves from infection.
Complete answer:
They can cut DNA at a particular location with a high level of explicitness. For example, a restriction protein named EcoR1 from the microbe E coli (the same microorganisms found in our stomach-related framework) cuts the DNA strand only where the GAATTC structure is available and nowhere else.
DNA Restriction cleavage catalysts are self-protection instruments for microorganisms. One of the most noticeably awful things that can happen to a bacterium is an intrusion by a bacteriophage infection. These infections infuse their DNA and can assume control over the cell so that as opposed to being the "parent" of 2 microorganisms, the bacterium turns into the "parent" of possibly 37 T2 phage infections! The best approach to stop that is to slash up the attacking DNA, and that is the thing that restriction enzymes should do.
It's also worth noting that these chemicals have no effect on bacterial DNA because the bacterial system methylates its own DNA, so these RE's have no effect on it. Because of the clarity they provide, REs are used in hereditary designing analyses where a specific site in the DNA is to be cut. These compounds are used for a variety of purposes, including high-quality cloning, hybridization, and DNA fingerprinting.
Note:
Viral particles attack bacterial creatures. Microorganisms use RESTRICTION ENDONUCLEASE catalysts to destroy viral DNA, implying that the compound is nucleic acids. Such chemicals do not act arbitrarily, but only in specific locations do they cut the DNA. Such destinations are short palindromic DNA groupings.
Complete answer:
They can cut DNA at a particular location with a high level of explicitness. For example, a restriction protein named EcoR1 from the microbe E coli (the same microorganisms found in our stomach-related framework) cuts the DNA strand only where the GAATTC structure is available and nowhere else.
DNA Restriction cleavage catalysts are self-protection instruments for microorganisms. One of the most noticeably awful things that can happen to a bacterium is an intrusion by a bacteriophage infection. These infections infuse their DNA and can assume control over the cell so that as opposed to being the "parent" of 2 microorganisms, the bacterium turns into the "parent" of possibly 37 T2 phage infections! The best approach to stop that is to slash up the attacking DNA, and that is the thing that restriction enzymes should do.
It's also worth noting that these chemicals have no effect on bacterial DNA because the bacterial system methylates its own DNA, so these RE's have no effect on it. Because of the clarity they provide, REs are used in hereditary designing analyses where a specific site in the DNA is to be cut. These compounds are used for a variety of purposes, including high-quality cloning, hybridization, and DNA fingerprinting.
Note:
Viral particles attack bacterial creatures. Microorganisms use RESTRICTION ENDONUCLEASE catalysts to destroy viral DNA, implying that the compound is nucleic acids. Such chemicals do not act arbitrarily, but only in specific locations do they cut the DNA. Such destinations are short palindromic DNA groupings.
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