Question

Explant is
A. Propagule
B. Callus used for subculturing
C. Part of plant used in tissue culture
D. Part of tissue used for planting

Answer 1

Hint: Plants are multicellular organisms. They are called autotrophs because they can make their own food with the help of a process called photosynthesis. Photosynthesis is a process by which plants make their own food with the help of Sunlight, Chlorophyll, water and Carbon Dioxide.

Complete Answer:
- An explant is a small part of the plant which is used to initiate tissue culture. The parts of plants such as stem and leaves are the most commonly used explants that are used for the tissue culture.
- Apart from the stem and leaves, other parts of plants such as flowers, roots, seed can also be used as explants for tissue culture.
- Explant culture is a technique which is used to culture cells from a piece of tissue or organ removed from the plants.
- The extraction and growing process of explanation should be sterilized due to which chances of infection get decreased.

Applications of explant culture:
- Explant culture is used to study organogenesis (formation of organs) and morphogenesis. This can be done by using fluorescent markers. Culture the explants in the lab and Use fluorescent markers at the area of interest. We will be able to study organogenesis and morphogenesis by observing these fluorescent markers.
- Explant culture is also useful for the study of the role of specific gene, gene expression and the mechanism of action .

Hence, option C is the right option for this question.

Note: Explant culture comes under the category of Plant tissue culture. In plant tissue culture we produce plants from the meristematic tissue of another plant under an artificial nutrient medium and environment. The plants produced by these techniques are exactly the clones of their mother in all aspects.