Question

Draw a neat labelled diagram of PRB322.

Answer 1

Hint: The recombination is one of the most used techniques in the biotechnology fields. It is used for the production of mixed featured organisms which have characters from different organisms. They can be recombined at the genetic level and introduced into a host to develop.

Complete answer:
Plasmid is the extrachromosomal DNA molecule which can independently replicate.
Cloning vectors is a vehicle used to artificially carry the foreign gene into a host. The gene can be expressed in the host. All the cloning vectors have an ori site, multi cloning site and selectable marker. The various cloning vectors used are- plasmid, cosmids, bacteriophages, YAC, BAC and phages.
PBR322 is a plasmid cloning vector. The P stands for Plasmid, BR for Bolivar and Rodiguez. They have 4361 base pairs. They have two antibiotic resistance genes- the ampicillin resistance protein and tetracycline resistance protein.
It has the origin of replication sites (ori). It has more than 40 restriction sites. This number is unique to every cloning vector. There are 11 sites in the tetracycline resistance site and 6 main restriction sites in the ampicillin restriction gene.
The genes of resistance can be amplified using the chloramphenicol. It is a double-stranded molecule.
EcoRI, HindIII and BamHI are some of the restriction enzymes on the PBR322.

Note: PBR322 was developed by Francisco Bolivar and Raymond Rodriguez. The 322 in PBR322 stands for the plasmid number to distinguish it from other plasmids. It is the first artificial cloning vector produced. There is ROP protein in the PRB322 for keeping the copy number of ColE1 low.