Question

Choose the correct answer from the alternatives given: The process of transformation is not affected by which of the following enzymes?
A. DNase, B. RNase, C. Peptidase, D. Lipase

A. A,B
B. A,B,C,D
C. B,C,D
D. A,B,C

Answer 1

Hint: Transformation is a process by which a foreign gene or DNA is inserted into the Bacteria. This property of bacteria is harnessed in the cloning. The genetically engineered plasmid is inserted into the bacteria by the process of cloning.

Complete Answer:
Transformation is the process of inserting a foreign DNA into the bacterial cell. This process takes place naturally but also can be carried out in the laboratory through a process called cloning. Avery-Macleod and McCarty through their experiment found out that DNA is the substance which caused bacterial transformation and not the proteins.

According to the question:
- Option A: DNase- this enzyme cleaves or destroys the DNA by breaking it into fragments. As DNA causes transformation, this enzyme is not used in transformation.
- Option B: RNase this enzyme cleaves or destroys the RNA by breaking it into fragments. This enzyme does not affect transformation.
- Option C: Peptidase- It is a proteolytic or protein digesting enzyme. So the transformation is unaffected.
- Option D: Lipase- It is a lipid digesting enzyme. So the transformation is unaffected.

Thus the correct answer is (C).

Note: Transformation is used to make multiple copies of DNA. It is also used to produce specific proteins like insulin, antibodies, and vaccines. It is a stable genetic change brought about by the help of naked DNA.