Question

cDNA is
A. Synthesized by reverse transcriptase
B. Cloned DNA
C. Circular DNA
D. Recombinant DNA

Answer 1

Hint: We can say that cDNA is one of the complementary DNA. This cDNA is used by organisms like viruses to convert viral RNA into mRNA. The cDNA is used in the formation of a cDNA library generally in gene probes or gene cloning. The methods like RT-qPCR or RNA sequence help in the study of gene expressions. It is made use of bioinformatics. A complementary DNA is formed to evaluate the transcriptomic profiles in bulk tissue in molecular biology.

Complete answer:
A messenger RNA template synthesizes complementary DNA in a reaction. This reaction is catalyzed by the enzymes reverse transcriptase and DNA polymerase.
-In prokaryotes, complementary DNA is used to clone eukaryotic genes.
-Eukaryotic genes consist of introns which are noncoding sequences, in disparity with exons, in which lines that code for mRNA.
-The synthesis of cDNA RNA serves as a template.
-Cloned DNA is a copy of a gene or other piece of DNA.
- Circular DNA forms a structure of a closed-loop and has no ends.
-The combination of molecules of DNA forms recombinant DNA from two different organisms that are inserted into a host organism to produce new genetic combinations.
So, the correct answer is “Option A”.

Additional Information:
Retroviruses can naturally produce cDNA by using their reverse enzyme transcriptase, and then it is integrated into the host's genome.
- Cloned DNA is required to investigate the gene's characteristics.
-cDNA is the DNA without all the necessary noncoding regions.
-cDNA is used instead of DNA because it has no introns.

Note:
cDNA is a complementary DNA. cDNA is synthesized from a messenger RNA template in a reaction. This reaction is catalyzed by the enzymes reverse transcriptase and DNA polymerase. If a specific protein in a cell is not expressed, then it is transferred to cDNA that codes for the protein to the recipient cell by scientists. In molecular biology, complementary DNA is formed to analyze transcriptomic profiles in bulk tissue, single nuclei in assays such as RNA- seq and microarrays.