Question

A technique of micropropagation is:
A. Somatic embryogenesis
B. Protoplast fusion
C. Embryo rescue
D. Somatic hybridization

Answer 1

Hint: With the collection of plant material to be propagated, micropropagation begins. In a sterile state, the plant tissues are separated from an intact plant. In the processing of the healthiest plants, clean stock materials that are free of viruses and fungi are important. The selection of explants in starts after the plant material is selected for culture .

Complete answer:
Often related to tissue culture, micropropagation is being used to multiply plants like those that have been genetically engineered or bred by traditional methods of plant breeding. It is often used to provide an appropriate number of plantlets that do not grow seeds or do not react well to vegetative reproduction from a stock plant for planting.

Now, let us find the solution from the options-
- The process of growing somatic embryos in vitro through the culture of somatic cells is somatic embryogenesis. The somatic cell-derived embryos are known as somatic embryos. Micropropagation is indeed the clonal propagation utilizing tissue culture; somatic embryogenesis is often a micropropagation procedure wherein somatic embryos are elevated by somatic cell culture.
- Protoplast fusion is a physical process. Two or even more protoplasts come into contact throughout fusion and bind to each other either spontaneously or in the existence of chemicals which induce fusion. Thus, option B is not the correct option.
- Embryo rescue is the procedure of embryonic development in vitro, as the embryos are expected to get abort early. Thus, option C is not the correct option.
- The development of hybrid plants by the merger of protoplasts of two distinct species as well as varieties of plants is somatic hybridization. Thus, option D is not the correct option.

Hence, the correct answer is option (A).

Note: There are three kinds of micropropagation strategies based on the way of propagation: firstly propagation of cytokinin shoots such as benzyladenine or kinetin; secondly, multiple shoot separation through auxin-like indole acetic acid from dedifferentiating tissue, callus; and eventually, the distinction of the embryo from callus.