A major use of embryo culture is in
A) Induction of soma clonal variation
B) Production of alkaloids
C) Clonal propagation.
D) Overcoming hybridization barriers

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Hint: Embryo culture is an in vitro culture technique that allows plants to overcome hybridization barriers. It is one of the earliest techniques used to assist the development of plant embryos, and these plant embryos may not survive to become viable plants. It can breed immature embryos. The zygote undergoes cell division and causes the formation of an embryo. The formation of embryos in the laboratory is called embryo culture.

Complete answer:
Embryo culture is also called embryo rescue. This technique was first recorded in the $18$ th century. It was discovered by Charles Bonnet. Hanning made its first successful attempt in $1904$ . Modern $1$ n plant breeding embryo culture plays an important role by allowing the development of many interspecific and interspecific foods.

The main uses of embryo rescue or culture are: Determine seed vigor, overcoming seed dormancy, used in plant physiology to study the germination and development of seeds, restore maternal haploid, it allows reproduction of incomplete inter-genus and interspecific species.

Hence the correct answer is option ‘D’.

Note: Nature does not allow interspecific plants to reproduce. To overcome this reproductive isolation, we use embryo culture. The hybridization of species takes place in a laboratory medium, and embryos of the desired species are produced by mixing the genetic pools of the two species. Embryo culture is a technique for culturing embryos under sterile conditions.